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Title: | Evaluation of leaves of Goniothalamus wynaadensis Bedd. for inhibition of metabolic viability of cancer cells & antimicrobial efficacy |
Authors: | Sharma A Sharma P Singh S Karegoudar T.B Holla H. |
Keywords: | Anticancer activity Antimicrobial activity Goniothalamus wynaadensis Bedd Phytochemical screening Survival index Turbidometric analysis |
Issue Date: | 2019 |
Publisher: | Elsevier GmbH |
Citation: | European Journal of Integrative Medicine , Vol. 32 , , p. - |
Abstract: | Introduction: Goniothalamus wynaadensis Bedd. is a less explored medicinal plant belonging to the family Annonaceae. This species has been traditionally used by tribes of Wayanad, Kerala, in India, and also Indonesian tribes for joint-related ailments and also as a deodorizer. The aim of the study was to analyze leaf extracts of the plant for their antimicrobial and anticancer properties along with phytochemical screening. Methods: MTT assay was used to assess the cytotoxic potential of plant extracts against MDA-MB-231 and A-549. Antimicrobial potential of plant extracts was analyzed via liquid broth turbidometry assay and well in agar plate methods. Identification tests were carried out for phytochemical screening. Results: Antimicrobial assay showed EC50 values for ethyl acetate extract 0.82 mg/mL against Escherichia coli, 0.82 mg/mL against Salmonella typhi, & 0.88 mg/mL against Staphylococcus aureus which are quite significant. Other solvent extracts also displayed antimicrobial activities at varying dosage. MTT assay was performed at the dose concentrations of 1 ?g/mL, 5 ?g/mL & 25 ?g/mL, 50 ?g/mL and 100 ?g/mL to confirm the cytotoxicity of the extracts. The ethyl acetate extract displayed EC50 values of 4.96 ?g/mL against A-549 lung cancer cell line and 2.50 ?g/mL against MDA-MB-231 breast cancer cell line. Other extracts were also found to be cytotoxic. Conclusion: Ethyl acetate & water extracts have demonstrated potent activity against Salmonella typhi, Escherichia coli, & Staphylococcus aureus bacteria as well as, showing cytotoxicity against tested cancer cell lines. These results can be explored to identify individual phyto-molecules from the extracts with anticancer & antimicrobial potential. © 2019 Elsevier GmbH |
URI: | 10.1016/j.eujim.2019.101000 http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4004 |
Appears in Collections: | 1. Journal Articles |
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