Spectrophotometric methods for the estimation of satranidazole in pharmaceutical formulations

Abstract

Two spectrophotometric methods (I and II) in the visible region have been developed for the estimation of satranidazole in bulk drug and pharmaceutical formulations. Methods I and II are based on the reaction of reduced satranidazole with p-dimethylaminobenzaldehyde (PDAB) and p-dimethylaminocinnamaldehyde (PDACA) in acidic conditions to form orange red and purple coloured chromogens with absorption maxima at 511 nm and 568 nm respectively.The reduction of satranidazole was carried out with zinc granules and 4 N hydrochloric acid at room temperature in ethanol. Beer's law was obeyed in the concentration range of 10-50 ?g/ml for both the methods. The results of analysis have been validated statistically and by recovery studies. The methods were found to be simple, rapid, accurate, reproducible and economic. The results are comparable with those obtained using UV spectrophotometric methods in alcohol at 315 nm.