Dac-elisa technique for early detection of red rot pathogen in sugarcane var. CoC 671

Abstract

The sugarcane variety CoC 671 is an early maturing, high sugar content variety in India. Recently, it has become very susceptible to red rot caused by Colletotrichum falcatum Went. To detect the pathogen at an early stage, a new serological technique, enzyme-linked immunosorbent assay was developed based on antiserum developed against the protein of host pathogen. Direct antigen coating enzyme-linked immunosorbent assay (Dac-Elisa) for the early detection of red rot infection has been standardized. Further, this technique was found reliable to screened planting material of sugarcane variety CoC 671 for red rot infection at an earlier stage. Besides, ill vitro red rot treated as well as healthy calli were also screened for red rot infection.