GULBARGA UNIVERSITY, KALABURAGI Institutional Repository
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http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5415| Title: | Cellulase-free thermostable alkaline xylanase from thermophilic and alkalophilic Bacillus sp JB-99 |
| Authors: | Johnvesly, B Virupakshi, S Patil, GN Ramalingam Naik, GR |
| Keywords: | Bacillus sp. biobleaching cellulase-free xylanase thermoalkalophilic |
| Issue Date: | 2002 |
| Publisher: | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY |
| Citation: | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY , Vol. 12 , 1 , p. 153 - 156 |
| Abstract: | The characterization of a partially purified, cellulase-free, thermostable alkaline xylanase from thermoalkalophilic Bacillus sp. JB-99 was investigated. The xylanase production was the highest when birchwood xylan was added to a medium containing finely powdered rice bran, showing 4,826 IU ml(-1) of activity for 15 h of incubation. The partially purified xylanase exhibited an optimum temperature and pH at 70degreesC and 10, respectively. The enzyme was stable at pH 5-11 at 50degreesC. The xylanase activity was strongly inhibited by Hg2+, while dithiothreitol, cysteine, and beta-mercaptoethanol enhanced the activity. |
| URI: | http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5415 |
| Appears in Collections: | 1. Journal Articles |
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