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http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5269| Title: | Purification and partial characterization of cytochrome c(552) from Halobacterium salinarium |
| Authors: | Sreeramulu, K |
| Keywords: | cytochrome c(552) Halobacterium salinarium purification characterization |
| Issue Date: | 2003 |
| Publisher: | NATL INST SCIENCE COMMUNICATION-NISCAIR |
| Citation: | INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS , Vol. 40 , 4 , p. 274 - 277 |
| Abstract: | Cytochrome C-552 was purified to near homogenity and partially characterized from Halobacterium salinarium JWS mutant, devoid of carotenoid pigments. The purification, involved the extraction of membranes with 1% Triton X-100, followed by butylagarose, DEAE-Sepharose CL6B and hydroxyapatite column chromatography. The fold of purification was 16. The purified cytochrome showed maximum absorption at 552 nm. The molecular mass determined by SDS-PAGE was found to be 14.1 kD. |
| URI: | http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5269 |
| Appears in Collections: | 1. Journal Articles |
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