GULBARGA UNIVERSITY, KALABURAGI Institutional Repository
Please use this identifier to cite or link to this item:
http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5228| Title: | A novel, rapid, inexpensive, and highly sensitive experiment for demonstration of DNA damage in human leukocytes by single cell gel electrophoresis (comet assay) |
| Authors: | Manjunatha B.R Manjunatha G.T.S Sarika C Roopa K.M Naik G.R. |
| Keywords: | Comet assay DNA damage Risk assessment Smokers |
| Issue Date: | 2003 |
| Citation: | Biochemistry and Molecular Biology Education , Vol. 31 , 1 , p. 34 - 36 |
| Abstract: | The single cell gel electrophoresis technique (comet assay) has been adopted to demonstrate the DNA damage in human leukocytes of individuals who are habitual smokers. This technique allows the detection of single strand and double strand DNA breaks, which are indicative of the risk of cancer. The method followed is rapid, inexpensive, and a highly sensitive technique for the evaluation of DNA damage and risk assessment in smokers. This experiment should help students understand the effect of cigarette smoking. © 2003 by The International Union of Biochemistry and Molecular Biology. |
| URI: | 10.1002/bmb.2003.494031010159 http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5228 |
| Appears in Collections: | 1. Journal Articles |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.