The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea

Abstract

A highly efficient protocol for plant regeneration from cotyledonary node of two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri used phenylacetic acid (PAA). The Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 6-benzylaminopurine (BAP) and 1.0 mg dm-3 PAA was used for induction of bud formation. Buds were elongated on MS medium supplemented either with only 0.75 mg dm-3 gibberellic acid (GA3) or 0.2 mg dm-3 GA3 + 0.6 mg dm-3 PAA. The elongated shoots were then transferred onto rooting medium containing 1 mg dm-3 PAA. The frequency of multiple shoot induction and rooting was higher in Annigeri as compared to ICCV-10. The complete plantlets with well-developed roots were transferred to pots containing sterilized soil and sand in the ratio 3:1 where they survived (74 %) and set normal seeds. © Springer Science+Business Media B.V. 2009.