Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5215
Title: The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea
Authors: Ghanti S.K
Sujata K.G
Rao M.S.
Keywords: Auxins
Cytokinins
Elongation
Gibberellic acid
Multiple shoots
Issue Date: 2009
Citation: Biologia Plantarum , Vol. 53 , 4 , p. 779 - 783
Abstract: A highly efficient protocol for plant regeneration from cotyledonary node of two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri used phenylacetic acid (PAA). The Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 6-benzylaminopurine (BAP) and 1.0 mg dm-3 PAA was used for induction of bud formation. Buds were elongated on MS medium supplemented either with only 0.75 mg dm-3 gibberellic acid (GA3) or 0.2 mg dm-3 GA3 + 0.6 mg dm-3 PAA. The elongated shoots were then transferred onto rooting medium containing 1 mg dm-3 PAA. The frequency of multiple shoot induction and rooting was higher in Annigeri as compared to ICCV-10. The complete plantlets with well-developed roots were transferred to pots containing sterilized soil and sand in the ratio 3:1 where they survived (74 %) and set normal seeds. © Springer Science+Business Media B.V. 2009.
URI: 10.1007/s10535-009-0143-7
http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5215
Appears in Collections:1. Journal Articles

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