Spectrophotometric determination of ornidazole

Abstract

Three simple, sensitive, selective and economical methods (method A, B and C) for the determination of ornidazole have been described. Method A, B and C are based on the formation of yellow, blood red and blue coloured chromogens when reduced ornidazole in situ reacted with vanillin, 1,10-phenanthroline in presence of ferric ions and Folin-Ciocalteau (FC) reagents exhibiting ?max at 426, 508.8 and 648 nm, respectively, which obeyed Beer's law in the concentration range of 5-25, 2-10 and 5-25 ?g/ml respectively. The results of analysis for the three methods have been validated statistically and by recovery studies. The results are comparable with those obtained with UV spectrophotometric method in alcohol at 312.8 nm.