Plant regeneration and in vitro flowering from leaf and nodal explants of Solanum nigrum (L.) - An important medicinal plant

Abstract

Direct organogenesis and in vitro flowering was obtained in Solanum nigrum (L.). High frequency and maximum number of multiple shoots were obtained from leaf and nodal explants on MS-medium supplemented with BAP and IAA (2.0mg/l + 1.0 mg/l). Regenerates, when transferred to rooting medium (0.5-2.0 mg/l IBA or IAA), they initiated flowering along with rooting. In vitro flowers set viable seeds. Rooted plantlets were hardened and transferred to polycups containing sterilized soil and vermiculate in the ratio 1:1 and subsequently transferred to green house with 95% survivability. This finding has a significant role in pharmaceutical industries to obtained solasodine and in vitro flowering facilitates in vitro pollination and fertilization, further it also facilitates in advancing the generation at much faster speed under limited progeny size in the segregating generation of Solanum nigrum (L.). © 2005 Society for Biology and Biotechnology.