Optimization of process for the production of fungal pectinases from deseeded sunflower head in submerged and solid-state conditions

Abstract

Pectinase production studies were carried out in submerged and solid-state conditions from deseeded sunflower head employing Aspergillus niger. The two potential strains of A. niger, DMF 27 for submerged and DMF 45 for solid-state were isolated by multi-step screening technique based on coefficient of pectolysis and capability of pectinase production. Process variables such as size of inoculum, pH, temperature, particle size and moisture content were optimized with an aim to achieve the maximum production of pectinases. The increased level of pectinase production was recorded at pH 5.0 and temperature 34 °C in submerged and solid-state conditions. The optimum inoculum size was 1 × 105 ml-1 for submerged and 1 × 107 g-1 for solid-state conditions. Five hundred micrometer particle size and 65% moisture content of the substrate were optimum for the maximum production of pectinases in solid-state condition. Under optimum conditions, maximum production of exo-pectinase was 34.2 U/g in SSF and endo-pectinase was 12.6 U/ml in SmF. © 2005 Elsevier Ltd. All rights reserved.