Development and validation of spectrophotometric methods for the determination of Cefetamet in pharmaceutical dosage forms

Abstract

Two simple and sensitive spectrophotometric methods for the determination of cefetamet in either pure form or in its pharmaceutical formulations were described. The method I is based on the interaction of 3-methylbenzo[d]- thiazolin-2-one hydrazone (MBTH) with Cefetamet in the presence of freshly prepared ferric chloride in a neutral medium. The resulting blue colored product has ?max at 628 nm. The method II describes the reduction of ferric ion by the drug to ferrous ion followed by a complex formation reaction with 1,10-phenanthroline (1,10-phen) to form an orange red colored chromogen exhibiting ?max at 510 nm. The products are stable for more than 5 and 8 h respectively. Common excipients used as additives in pharmaceutical preparations do not interfere in the proposed methods. Both methods are highly reproducible and have been applied to a wide variety of pharmaceutical preparations and the results are comparable with those of official methods. © 2007 SIOC, CAS, & Wiley-VCH Verlag GmbH & Co. KGaA.