Saccharomonospora saliphila sp. nov., a halophilic actinomycete from an Indian soil

Abstract

A Gram-positive, moderately halophilic actinomycete strain, designated YIM 90502T, was isolated from a sample of muddy soil collected from Gulbarga, Karnataka Province, India, and subjected to a polyphasic taxonomical analysis. The isolate grew optimally at 28 °C and in the presence of 10 % (w/v) NaCl. The isolate was characterized chemotaxonomically as having meso-diaminopimelic acid in the cell-wall peptidoglycan and galactose and arabinose as whole-cell sugars. The predominant menaquinone was MK-9(H4), while MK-8(H4) was found in smaller amounts. The phospholipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C16:0 (49.2 %) and C17:1?6c (9.1 %). The DNA G + C content of the isolate was 71.8 mol%. A phylogenetic tree based on 16S RNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus Saccharomonospora. 16S rRNA gene sequence similarity between strain YIM 90502T and the type strains of Saccharomonospora species ranged from 92.42% (with Saccharomonospora xinjiangensis CCTCC AA 97021T) to 97.45% (with Saccharomonospora azurea KCTC 9693T). Levels of DNA-DNA relatedness between strain YIM 90502T and S. azurea KCTC 9693T, Saccharomonospora halophila DSM 44411T and Saccharomonospora paurometabolica DSM 44619T were 46.0, 41.0 and 42.5%, respectively. On the basis of phenotypic, phylogenetic and genotypic data, strain YIM 90502T was classified in the genus Saccharomonospora as a member of a novel species, for which the name Saccharomonospora saliphila sp. nov. is proposed, with YIM 90502T (=KCTC 19234T =DSM 45087T) as the type strain. © 2008 IUMS.