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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dhananjay S.K | |
dc.contributor.author | Mulimani V.H. | |
dc.date.accessioned | 2020-06-12T15:05:27Z | - |
dc.date.available | 2020-06-12T15:05:27Z | - |
dc.date.issued | 2008 | |
dc.identifier.citation | Biotechnology Letters , Vol. 30 , 9 , p. 1565 - 1569 | en_US |
dc.identifier.uri | 10.1007/s10529-008-9722-5 | |
dc.identifier.uri | http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4858 | - |
dc.description.abstract | ?-Galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). ?-Galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE. © 2008 Springer Science+Business Media B.V. | en_US |
dc.subject | ?-Galactosidase | |
dc.subject | Aspergillus oryzae | |
dc.subject | Purification | |
dc.subject | Tert-Butanol | |
dc.subject | Three-phase partitioning | |
dc.title | Purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae | en_US |
dc.type | Article | |
Appears in Collections: | 1. Journal Articles |
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