Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4858
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dc.contributor.authorDhananjay S.K
dc.contributor.authorMulimani V.H.
dc.date.accessioned2020-06-12T15:05:27Z-
dc.date.available2020-06-12T15:05:27Z-
dc.date.issued2008
dc.identifier.citationBiotechnology Letters , Vol. 30 , 9 , p. 1565 - 1569en_US
dc.identifier.uri10.1007/s10529-008-9722-5
dc.identifier.urihttp://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4858-
dc.description.abstract?-Galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). ?-Galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE. © 2008 Springer Science+Business Media B.V.en_US
dc.subject?-Galactosidase
dc.subjectAspergillus oryzae
dc.subjectPurification
dc.subjectTert-Butanol
dc.subjectThree-phase partitioning
dc.titlePurification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzaeen_US
dc.typeArticle
Appears in Collections:1. Journal Articles

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