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http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4858| Title: | Purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae |
| Authors: | Dhananjay S.K Mulimani V.H. |
| Keywords: | ?-Galactosidase Aspergillus oryzae Purification Tert-Butanol Three-phase partitioning |
| Issue Date: | 2008 |
| Citation: | Biotechnology Letters , Vol. 30 , 9 , p. 1565 - 1569 |
| Abstract: | ?-Galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). ?-Galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE. © 2008 Springer Science+Business Media B.V. |
| URI: | 10.1007/s10529-008-9722-5 http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4858 |
| Appears in Collections: | 1. Journal Articles |
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