ISSR Analysis of chloroplast and mitochondrial genome can indicate the diversity in sugarcane genotypes for red rot resistance

Abstract

Organellar genome inter-simple sequence repeat markers were used to analyze the red rot disease and its genetic relationship in 12 widely cultivated elite sugarcane (Saccharum spp. hybrid) genotypes. Out of 12 cultivars selected for screening, seven were resistant/moderately resistant and remaining 5 susceptible to red rot disease. cpISSR loci showed 56.7% polymorphism (67 fragments scored from 12 primers tested), while 66.6% polymorphism was observed for mtISSR loci (72 fragments scored from 12 primers tested). The genetic diversity for chloroplast DNA and mitochondrial DNA was 0.17-0.76 and 0.16-0.64 respectively, which showed significant level of polymorphism among the genotypes. The dendrogram produced by Unweighted Pair Group Method with Arithmetic averages (UPGMA) clearly clustered red rot disease resistant/moderately resistant cultivars in one group and susceptible cultivars in another group. The results indicate that the cplSSR and mtISSR marker are a new powerful tool for the identification of disease resistant cultivars, their genetic relationship analysis and diversity evaluation. © 2008 Society for Sugar Research & Promotion.