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http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4769| Title: | Production and partial purification of Alpha-amylase from a novel isolate Streptomyces gulbargensis |
| Authors: | Syed D.G Agasar D Pandey A. |
| Keywords: | Amylolytic enzymes pH and temperature effect Purification Streptomyces |
| Issue Date: | 2009 |
| Citation: | Journal of Industrial Microbiology and Biotechnology , Vol. 36 , 2 , p. 189 - 194 |
| Abstract: | Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45°C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating ?-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa. © Society for Industrial Microbiology 2008. |
| URI: | 10.1007/s10295-008-0484-9 http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4769 |
| Appears in Collections: | 1. Journal Articles |
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