Simple laboratory exercise for induction of Beta-Mannanase from aspergillus niger

Abstract

This laboratory experiment was designed for Biochemistry, Biotechnology, Microbiology, and Food Technology students of undergraduate and postgraduate courses. The experiment shows the advantages of using agricultural waste, copra mannan as potent inducer of ?-mannanase. The students were able to compare the enzyme induction by commercial mannans (locust bean gum, and guar gum), copra meal, defatted copra meal, and monosaccharides (glucose, galactose, mannose, and xylose). The students appreciated the use of cheap, locally available copra meal for the enhanced production of enzyme. © 2009 Institute of Food Technologists®.