Screening of Beta-fructofuranosidase producers with high transfructosylation activity and its 32 experimental run studies on reaction rate of enzyme

Abstract

?-fructofuranosidases are a group of hydrolytic and transfructosylating enzymes that play an important role in food processing industry to synthesize Fructo Oligosaccharides (FOS). The present study aimed to screening of filamentous fungi strains and growing them in batch cultures to compare their abilities in production of ?-Fructofuranosidase (?-FFase) using sole carbon source as sucrose. Radial diffusion method was used to evaluate the potent ?-fructofuranosidase producers by using crude enzyme. Among screened strains, Aspergillus' niger PSSF21 (2.20±0.100 cm), Ckrysonilia sitopkila PSSF84 (2.00±0.026 cm) and Aspergillusflavus PSSF22 (1.64±0.040 cm) showed significant hydrolysis zone in the plate. In addition, the ? fructofuranosidase of the above strains also showed high fructosyltransferase activity to hydrolytic ratio (Aspergillus niger PSSF21-6.335 U mg_1, Ckrysonilia sitopkila PSSF84 3.487 U mg_1, Aspergillus flavus PSSF22-3.256 Umg-1). The effects of pH and temperature on the sucrose- ?-FFase reaction rate were studied using a 32 experimental design matrix. Acidic pH influences positively on the fructosyl transferase activity of three isolated strains, where as effect of temperature showed remarkable variations on high fructosyltransferase activity-to-hydro lytic activity ratio. © 2010 Asian Network for Scientific Information.