Regeneration Studies using Nodal Explant in Balanites aegyptiaca (L.) Del. -An Endangered Medicinal Plant

Abstract

In vitro studies were initiated for developing an efficient protocol callus induction and direct regeneration using different explants of Balanites aegyptiaca (L.) Del. Callus induction and direct regeneration of shoots was observed on MS basal medium supplemented with various concentrations of auxins and cytokinins. Callus induction was higher in leaf explants compared with other explants. Leaf explant produced maximum amount of callus on MS medium supplemented with 1.0 mg/l 2, 4-D + 1.0 mg/l BAP compared to other combinations used. Multiple shoots were induced from nodal explants on MS medium fortified with 1.5 mg/l BAP (10 2 per culture) and subsequent rhizogenesis was achieved on MS + 0.5 mg/l IBA.