Identification of multi-resistant staphylococcus aureus in clinical specimens

Abstract

In the present study, highly resistant S. aureus were isolated. Identification of multiple genes (mecA, ermA, vanA) for 5 multi-resistant 5. aureus strains have been attempted. These isolates contain 533 bp mecA fragment, 139 bp ermA fragment and 1030 bp vanA fragment by multiplex PCR method. Also antimicrobial susceptibility testing by disk diffusion method was evaluated for 360 S. aureus isolates against 20 antimicrobial agents. Among the 360 isolates, 117 isolates were resistant to >14 antimicrobial agents. The prudent use of antibiotics and rapid and continuous screening for resistant microorganisms should be more focused to prevent the emergence and spread of multi resistant MRSA strains. © Medwell Journals, 2010.