Toxicity studies on hepatic, nephric and endocrine organs of Citrus medica seeds extract on of female albino mice

Abstract

The present study is an evaluation to determine the toxicity level of benzene and ethanol extracts of Citrus medica seeds in the endocrine organs of female albino Swiss albino mice (Mus musculus) at the dose level of 200mg and 400mg/kg body weight for 15 days. The results exposed that the (administered of extract of C. medica seeds to female mice observed) changes in the gain/loss of body weight, organ weight (Liver, Kidney, Adrenal, Ovary and Uterus) morphological and behavioural studies. The detection of haematotoxicity, haemoglobin concentration, total RBC, WBC counts and total differential count were evaluated. The biochemical (Blood Sugar, Serum Cholesterol, Total Protein and Blood Urea Nitrogen) and histological changes in the liver, ovary and uterus were also determined. All the parameters of benzene extract treated mice were found to be within normal range, when compare to control. The data suggest that the ethanol extract of C. medica seeds at both the dose level administration for 15 days little effective and shown almost significant level of toxicity on liver as well as reproductive organs. © 2009, JGPT.