Production of L-glutaminase by Streptomyces rochei detected from the lime stone quarries of deccan trap

Abstract

The present study focuses on exploration of an actinomycete from limestone quarries of deccan trap for the production of L-glutaminase. Streptomyces rochei, a promising producer of L-glutaminase was confirmed based on 16S rDNA sequence (DMQ-14: JQ889270) analysis. At optimum level of process parameters (pH-8.0; temperature -40°C ; inoculum size-1×108 spores/ml and agitation-200 rev/min.) a linear increase (9.86±0.025 IU; 12.28±0.010 IU; 13.46±0.015 IU; 15.78±0.02 IU respectively) in the production of L-glutaminase by Streptomyces rochei was achieved. Among the nutritional sources, Starch and L-glutamine at 1.0% w/v proven to be the best carbon and nitrogen sources for the enhanced production (25.42±0.040 IU and 30.24±0.01 IU respectively) of L-glutaminase. MgSO4, 7H2O (0.05% w/v) proved to be the most suitable metal ion for further increase (31.55±0.020 IU) in the production of L-glutaminase. Thus, with all the optimized conditions, the maximum production of L-glutaminase was 31.55±0.020 IU.