Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4498
Title: Production of L-glutaminase by Pseudomonas VJ-6
Authors: Jyothi H
Kumar S
Vandana R.
Keywords: L-glutaminase
Pseudomonas sps.
Submerged fermentation
Issue Date: 2011
Citation: Research Journal of Biotechnology , Vol. 6 , 3 , p. 42 - 49
Abstract: Of the fifteen bacterial isolates screened for glutaminase production, four were positive for glutaminase production. Among them only one isolate Pseudomonas-VJ6 was selected based on plate assay. Glutaminase production was 51.5 U/ml by submerged fermentation. Enzyme activity of was assayed in various ranges of incubation period, temperature and pH. The enzyme activity was optimal at 72 hrs (53.3 U/ml), temperature, 30°C (56.0 U/ml) and pH of 6.0 (57.6 U/ml). Nutritional parameters, carbon and nitrogen sources also played an efficient role in enhancing the yield. Among the different carbon sources, glucose with concentration (10 g/l) showed maximum activity of 58.4 U/ml and among different nitrogen sources yeast extract (2 g/l) showed maximum activity of 58.9 U/ml. L-glutaminase was partially purified from cell free extracts of Pseudomonas-VJ6 by ammonium sulphate salt precipitation which showed activity of 56.6 U/ml at 80% precipitation and dialyzed enzyme extract having activity of 56.0 U/ml with protein content of 70 mg/ml was obtained. SDS-PAGE showed a molecular weight of 31 kDa. L-glutaminase from bacterial sources is reported to have high industrial application for their stability.C (56.0 U/ml) and pH of 6.0 (57.6 U/ml). Nutritional parameters, carbon and nitrogen sources also played an efficient role in enhancing the yield. Among the different carbon sources, glucose with concentration (10 g/l) showed maximum activity of 58.4 U/ml and among different nitrogen sources yeast extract (2 g/l) showed maximum activity of 58.9 U/ml. L-glutaminase was partially purified from cell free extracts of Pseudomonas-VJ6 by ammonium sulphate salt precipitation which showed activity of 56.6 U/ml at 80% precipitation and dialyzed enzyme extract having activity of 56.0 U/ml with protein content of 70 mg/ml was obtained. SDS-PAGE showed a molecular weight of 31 kDa. L-glutaminase from bacterial sources is reported to have high industrial application for their stability.
URI: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4498
Appears in Collections:1. Journal Articles

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