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Title: | Production of L-glutaminase by Pseudomonas VJ-6 |
Authors: | Jyothi H Kumar S Vandana R. |
Keywords: | L-glutaminase Pseudomonas sps. Submerged fermentation |
Issue Date: | 2011 |
Citation: | Research Journal of Biotechnology , Vol. 6 , 3 , p. 42 - 49 |
Abstract: | Of the fifteen bacterial isolates screened for glutaminase production, four were positive for glutaminase production. Among them only one isolate Pseudomonas-VJ6 was selected based on plate assay. Glutaminase production was 51.5 U/ml by submerged fermentation. Enzyme activity of was assayed in various ranges of incubation period, temperature and pH. The enzyme activity was optimal at 72 hrs (53.3 U/ml), temperature, 30°C (56.0 U/ml) and pH of 6.0 (57.6 U/ml). Nutritional parameters, carbon and nitrogen sources also played an efficient role in enhancing the yield. Among the different carbon sources, glucose with concentration (10 g/l) showed maximum activity of 58.4 U/ml and among different nitrogen sources yeast extract (2 g/l) showed maximum activity of 58.9 U/ml. L-glutaminase was partially purified from cell free extracts of Pseudomonas-VJ6 by ammonium sulphate salt precipitation which showed activity of 56.6 U/ml at 80% precipitation and dialyzed enzyme extract having activity of 56.0 U/ml with protein content of 70 mg/ml was obtained. SDS-PAGE showed a molecular weight of 31 kDa. L-glutaminase from bacterial sources is reported to have high industrial application for their stability.C (56.0 U/ml) and pH of 6.0 (57.6 U/ml). Nutritional parameters, carbon and nitrogen sources also played an efficient role in enhancing the yield. Among the different carbon sources, glucose with concentration (10 g/l) showed maximum activity of 58.4 U/ml and among different nitrogen sources yeast extract (2 g/l) showed maximum activity of 58.9 U/ml. L-glutaminase was partially purified from cell free extracts of Pseudomonas-VJ6 by ammonium sulphate salt precipitation which showed activity of 56.6 U/ml at 80% precipitation and dialyzed enzyme extract having activity of 56.0 U/ml with protein content of 70 mg/ml was obtained. SDS-PAGE showed a molecular weight of 31 kDa. L-glutaminase from bacterial sources is reported to have high industrial application for their stability. |
URI: | http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4498 |
Appears in Collections: | 1. Journal Articles |
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