Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4474
Title: Inhibition of P-glycoprotein ATPase and its transport function of Helicoverpa armigera by morin, quercetin and phloroglucinol
Authors: Aurade R.M
Akbar S.M.D, Goud H
Jayalakshmi S.K
Sreeramulu K.
Keywords: Drug transport
Flavonoids
Helicoverpa armigera
P-gp ATPase
Proteoliposome
Tryptophan quenching
Issue Date: 2011
Citation: Pesticide Biochemistry and Physiology , Vol. 101 , 3 , p. 212 - 219
Abstract: Flavonoids (morin, quercetin and phloroglucinol) were tested for their ability to modulate the function of P-glycoprotein ATPase of the insecticide resistant pest Helicoverpa armigera (Ha-Pgp). Flavonoids in the presence of ethylparaoxon or cypermethrin significantly reduced both larval weight as well as survival rate 40-50%. Morin and quercetin inhibited the activity of Ha-Pgp ATPase by 80-90%, whereas phloroglucinol inhibited ATPase activity by 40% at 100?M concentration. These flavonoids inhibited the verapamil, ethylparaoxon and cypermethrin-stimulated Ha-Pgp ATPase activity. Morin, quercetin and phloroglucinol binding were quantitated by quenching of the intrinsic Trp fluorescence of purified Ha-Pgp ATPase. Drug transport was monitored in proteoliposomes containing Ha-Pgp ATPase using the high affinity fluorescent substrate tetramethylrosamine (TMR) in real time. Addition of the morin and quercetin mediated the collapse of the TMR concentration gradient generated by Ha-Pgp ATPase. The inhibition studies on Ha-Pgp ATPase activity may contribute towards understanding new strategies of the pest to overcome insecticide resistance. © 2011 Elsevier Inc..
URI: 10.1016/j.pestbp.2011.09.008
http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4474
Appears in Collections:1. Journal Articles

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