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Title: | Inhibition of P-glycoprotein ATPase and its transport function of Helicoverpa armigera by morin, quercetin and phloroglucinol |
Authors: | Aurade R.M Akbar S.M.D, Goud H Jayalakshmi S.K Sreeramulu K. |
Keywords: | Drug transport Flavonoids Helicoverpa armigera P-gp ATPase Proteoliposome Tryptophan quenching |
Issue Date: | 2011 |
Citation: | Pesticide Biochemistry and Physiology , Vol. 101 , 3 , p. 212 - 219 |
Abstract: | Flavonoids (morin, quercetin and phloroglucinol) were tested for their ability to modulate the function of P-glycoprotein ATPase of the insecticide resistant pest Helicoverpa armigera (Ha-Pgp). Flavonoids in the presence of ethylparaoxon or cypermethrin significantly reduced both larval weight as well as survival rate 40-50%. Morin and quercetin inhibited the activity of Ha-Pgp ATPase by 80-90%, whereas phloroglucinol inhibited ATPase activity by 40% at 100?M concentration. These flavonoids inhibited the verapamil, ethylparaoxon and cypermethrin-stimulated Ha-Pgp ATPase activity. Morin, quercetin and phloroglucinol binding were quantitated by quenching of the intrinsic Trp fluorescence of purified Ha-Pgp ATPase. Drug transport was monitored in proteoliposomes containing Ha-Pgp ATPase using the high affinity fluorescent substrate tetramethylrosamine (TMR) in real time. Addition of the morin and quercetin mediated the collapse of the TMR concentration gradient generated by Ha-Pgp ATPase. The inhibition studies on Ha-Pgp ATPase activity may contribute towards understanding new strategies of the pest to overcome insecticide resistance. © 2011 Elsevier Inc.. |
URI: | 10.1016/j.pestbp.2011.09.008 http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4474 |
Appears in Collections: | 1. Journal Articles |
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