Human alpha amylase inhibitor activity by polyphenolic extracts of mung bean cultivars (Phaseolus aureus L.)

Abstract

Ten cultivars of mung bean (Phaseolus aureus L.) including three yellow cultivars differing in seed color were analyzed for their polyphenol content. The effect of polyphenolic extract on human salivary alpha amylase (HSA) activity was evaluated. The total levels of polyphenol were found to be in the range of 280 mg to 356 mg/100 gm whole seeds. The polyphenol content of yellow cultivars was relatively more with that of green cultivars except China mung. About 75% of polyphenols were extracted with acidic methanol while only 25% with methanol. The highest HAS inhibitory activity of 42.55 units g-1 was found with acidic methanol extract of ALM-1 cultivar, while the cultivar PS-16 had the lowest 24.09 units g-1 HSA inhibitory activity. The inhibition of alpha amylase by acidic methanol extracts of mung bean was found to be in the range of 48.50% to 64.63%. No correlation was observed between the polyphenol content and HAS inhibitory activity. The inhibition of human salivary amylase by mung bean polyphenolic extracts is significant strategy for managing postprandial glycemia in diabetic patients.