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DC Field | Value | Language |
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dc.contributor.author | Aurade R.M | |
dc.contributor.author | Jayalakshmi S.K | |
dc.contributor.author | Udikeri S.S | |
dc.contributor.author | Sreeramulu K. | |
dc.date.accessioned | 2020-06-12T15:03:39Z | - |
dc.date.available | 2020-06-12T15:03:39Z | - |
dc.date.issued | 2012 | |
dc.identifier.citation | Archives of Insect Biochemistry and Physiology , Vol. 79 , 1 , p. 47 - 60 | en_US |
dc.identifier.uri | 10.1002/arch.21004 | |
dc.identifier.uri | http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4348 | - |
dc.description.abstract | Purified P-glycoprotein ATPase from Helicoverpa armigera (Ha-Pgp), reconstituted in proteoliposomes composed of phospholipids and cholesterol, shows higher ATPase activity in the presence of cholesterol than in its absence. The Ha-Pgp ATPase activity was increased 30-40% with cholesterol. The KM for ATP was found to be 1 and 0.8 mM in the absence and presence of cholesterol, respectively. The insecticide-stimulated Ha-Pgp ATPase activity was increased by 10-20% for all the insecticides in the reconstituted proteoliposomes containing cholesterol compared to those with no cholesterol. The effects of cholesterol on KM and Vmax values of insecticide-stimulated Ha-Pgp ATPase activity were unrelated to the size of the insecticide. Ha-Pgp tryptophan fluorescence displayed a red shift of 3 and 8 nm in emission spectra upon binding of insecticides. Cholesterol enhances the interaction of insecticides with Ha-Pgp. Kd values of different insecticides for binding to Ha-Pgp were found to be lower in the presence of cholesterol in the proteoliposomes compared to its absence. Results suggest that cholesterol plays a role in the recognition and interaction of insecticides by modulating Ha-Pgp ATPase and may be involved in efflux of insecticides from cells by the transporter. © 2011 Wiley Periodicals, Inc. | en_US |
dc.subject | Cholesterol | |
dc.subject | Helicoverpa armigera | |
dc.subject | Insecticides | |
dc.subject | P-glycoprotein atpase | |
dc.subject | Tryptophan quenching | |
dc.title | Modulation of P-glycoprotein atpase of helicoverpa armigera by cholesterol: Effects on atpase activity and interaction of insecticides | en_US |
dc.type | Article | |
Appears in Collections: | 1. Journal Articles |
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