Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4294
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dc.contributor.authorHalkai K.R
dc.contributor.authorMudda J.A
dc.contributor.authorShivanna V
dc.contributor.authorRathod V
dc.contributor.authorHalkai R.
dc.date.accessioned2020-06-12T15:02:58Z-
dc.date.available2020-06-12T15:02:58Z-
dc.date.issued2018
dc.identifier.citationContemporary Clinical Dentistry , Vol. 9 , 2 , p. 237 - 241en_US
dc.identifier.uri10.4103/ccd.ccd_828_17
dc.identifier.urihttp://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4294-
dc.description.abstractAim: This study aims to evaluate the antibacterial efficacy of biosynthesized silver nanoparticles (AgNPs) produced using the fungi against Enterococcus faecalis biofilm model on root dentin. Materials and Methods: AgNPs were biosynthesized using the fungi Fusarium semitectum isolated from healthy leaves of Withania somnifera. Minimum inhibitory concentration (MIC) of AgNPs was determined by microbroth dilution method using series of dilutions. MIC dose was standardized to evaluate the antibacterial efficacy. For biofilm model, thirty root dentin blocks prepared using human extracted single-rooted teeth were inoculated with E. faecalis in Trypticase soy agar broth for 2 weeks with alternate day replenishment and randomly divided into three groups (n = 10 each) and treated as: Group I: Sterile distilled water, Group II: AgNPs, and Group III: 2% chlorhexidine gluconate (CHX) and incubated at 37°C for 24 h. Each dentin block was rinsed in saline, vortex shaken for 60 s, and serial decimal dilutions were prepared and plated on trypticase soy agar plates and incubated for 24 h followed by CFU colony counting and statistically analyzed using one-way ANOVA followed by post hoc Tukey honestly significant difference test. Results: MIC of AgNPs for E. faecalis was determined as 30 mg/ml. No significant difference was seen between AgNPs and 2% CHX when compared to the control group with mean colony counts being 2.4, 2.5, and 6.77 CFU/ml (107), respectively (P < 0.0001), against E. faecalis biofilm. Conclusion: Biosynthesized AgNPs exhibit effective antimicrobial activity against E. faecalis biofilm on root dentin. Therefore, it can be employed as antimicrobial agent for root canal disinfection. © 2018 Journal of Cancer Research and Therapeutics | Published by Wolters Kluwer - Medknow.en_US
dc.publisherWolters Kluwer Medknow Publications
dc.subjectAntimicrobial agent
dc.subjectbiofilm
dc.subjectendodontic disinfection
dc.subjectendophytic fungi
dc.subjectresistant microbe
dc.titleAntibacterial efficacy of biosynthesized silver nanoparticles against Enterococcus faecalis Biofilm: An in vitro studyen_US
dc.typeArticle
Appears in Collections:1. Journal Articles

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