Evaluation of antibacterial and antioxidant activities of different methanol extract of Rumex vesicarius L

Abstract

This study is designed to examine the antibacterial and antioxidant activity along with total phenolic content and flavonoid content of different extract i.e., WPH (whole plant hot), WPC (whole plant cold), LH (leaves hot) and LC (leaves cold) of Rumex vesicarius L. Antibacterial activity of all the extract was evaluated against five clinically significant bacterial cultures by agar well diffusion method. The different extract of R.vesicarius L. exhibited moderate to potent broad spectrum antibacterial activities targeting both gram+ve and gram-ve bacteria. The inhibition zone, relative% inhibition, MIC and MIB showed highly significant variations according to variations of extraction method, plant part and tested bacterial isolates. The WPH extract was found to be most effective against Pseudomonas aeruginosa (11.66±0.41), Staphylococcus aureus (9.33±0.577) and Escherichia coli (8.00±0.00), respectively, WPC showed the moderate activity against Pseudomonas aeruginosa (7±0.00). The LH extract exhibited significant activity against Escherichia coli (12.33±0.417) and Staphylococcus aureus (11.750±0.404) respectively and LC showed good antibacterial activity against Pseudomonas aeruginosa (10.33±0.932). The highest MIC value is 250 mg mL-1 and the lowest MIC value is found to be 31.25 mg mL-1. The MIC index calculated for each extract shows that MBC/MIC<4 thus all the extract of R.vesicarius are bactericidal. Antioxidant activities of the samples were determined by three different test systems, DPPH, lipid peroxidation, reducing power assay. In all the systems, WPH and LH extract exhibited excellent activity potential than those of other extract. A positive correlation was observed between the pharmacological activity and phenolic, flavonoid levels of the extract. © 2013 Academic Journals Inc.