Screening of streptomyces and process optimization for the production of tyrosinase

Abstract

The present study explains the extracellular production of tyrosinase by Streptomyces in submerged fermentation (SmF). A total of 12 isolates of streptomyces were screened for the qualitative and quantitative synthesis of tyrosinase in STM (starch tyrosine mineral) and TGB (tyrosine gelatin beef extract) media. Streptomyces DSV 12 was screened and selected to be an efficient strain for the maximum production (98.1 IU) of tyrsoinase in TGB medium. The enhanced production of tyrosinase by process optimization was carried out with physical and nutritional parameters. In Physical parameters the optimum conditions for maximum production (112.4 IU) were pH-7.5, temperature-40°C and aeration-180rpm. Further, enhanced production of tyrosinase was achieved with nutritional, such as carbon and nitrogen sources. Among all nutritional sources tested, glucose (0.5%) has shown higher production (120.7 IU) followed by L-tyrosine (0.5%) with maximum (125.4 IU) production. CuSO4 (0.06%)was proved to be the best inducer among all metal ions tested for the highest production (158.6 IU) of extracellular tyrosinase.