Evaluation of in vitro anti-thrombolytic activity and cytotoxicity potential of Typha angustifolia l leaves extracts

Abstract

Objective: The aim of this study was to investigate the in vitro thrombolytic activity and cytotoxicity of T angustifolia leaf extract. Methods: An in vitro thrombolytic model was used to evaluate the clot lysis effect of different extracts of T.angustifolia Linn along with Streptokinase as a positive control and distilled water as a negative control. The cytotoxic activity of diffetent extracts of T.angustifolia leaves was evaluated by Brine Shrimp Lethality Bioassay. Results: An in vitro thrombolytic model of aqueous, methanol and chloroform extracts have shown 51.76±2.5%, 58±2.32 and 18±1.84 clot lysis respectively, where as the positive control Streptokinase shown 79.6±1.10 and negative control shown negligible 2.44±0.62. The aqueous, methanol and chloroform extracts have shown brine shrimp lethality with LC50 value of 40?g/ml, 30?g and 104 ?g/ml respectively. It was found that aqueous and methanol extracts of T angustifolia possesses potential thrombolytic activity as well as cytotoxicity. Conclusion: The present investigation revealed that the aqueous and methanol extracts of T angustifolia possesses thrombolytic properties as well as cytotoxicity effects and it can be further used for treatment of cardiovascular diseases and cancer.