Nocardioides ginkgobilobae sp. Nov., an endophytic actinobacterium isolated from the root of the living fossil ginkgo biloba L

Abstract

A Gram-stain-positive, aerobic and yellow actinobacterial strain, designated SYP-A7303T, was isolated from the root of Ginkgo biloba L. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain SYP-A7303T belongs to the genus Nocardioides. The 16S rRNA gene sequence of strain SYP-A7303T showed highest similarity to Nocardioides marinus CL-DD14T (=JCM 15615T) (98.3%) and Nocardioides aquiterrae GW-9T (=JCM 11813T) (97.1%), and less than 96.9% to the type strains of other species of the genus Nocardioides. Strain SYP-A7303T grew optimally at 28 °C, pH 7.0 and in the absence of NaCl. It contained LL-2,6-diaminopimelic acid in the cell-wall peptidoglycan, with mannose, ribose, rhamnose, glucose and galactose as whole-cell sugars. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown lipid. The menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16: 0, C18: 1?9c and C17: 1?8c. The DNA G+C content was 72 mol%. Mean DNA-DNA relatedness values between strain SYP-A7303T and the closely related strains N. marinus JCM 15615T and N. aquiterrae JCM 11813T were 62.5±2.4 and 56.5±3.5%, respectively. Based on the morphological, physiological, biochemical and chemotaxonomic characteristics presented in this study, strain SYP-A7303T represents a novel species of the genus Nocardioides, for which the name Nocardioides ginkgobilobae sp. nov. is proposed. The type strain is SYP-A7303T (=DSM 100492T=KCTC 39594T). © 2016 IUMS.