Effect of growth regulators on regeneration through leaf and stem derived callus in Physalis minima Linn

Abstract

Physalis minima L. is a medicinal herb which belongs to the family Solanaceae. A rapid protocol by using leaf and stem derived callus culturesfor organogenesis and subsequent plantlet regeneration and survival of regenerated plantswas established.Murashige and Skoog’s (MS) basal medium supplemented with various concentrations of auxins like2,4- dichlorophenoxyacetic acid (1 to 3 mg/l), ánaphthaleneacetic acid (1 to 3 mg/l) and cytokinins like 6-benzylaminopurine (BAP)and kinetin (Kn) (0.5 to 1.5mg/l) were used for morphogenesis.Both the explants responded for callus induction, however, stem explants werefound superior than that of leaf. Further increase in callus was observed when0.5mg/l Kn was supplemented along with 2,4-D. The frequency of callus induction was 90%. It was noticed that among leaf and stem, leaf explants responded well for regenerationvia callus. The highest(95%) percentage of regeneration was recorded on MS medium containing1 mg/l BAP +2 mg/l 2,4-D from leaf derived callusafter a period of 20 to 25 days.Shootsof about 2 to 3 cm in height were subsequently rooted on MS medium supplemented with 1 mg/lindole-3- butyric acid (IBA),with 85% frequency. Rooted shoots were hardened in liquid half strength MS medium containing 1 mg/l IBA and acclimatized later in the pots containing soil, cocopeat and sand in the ratio 1:2:1 in the Net House. © 2016, Association of Biotechnology and Pharmacy. All rights reserved.