Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5549
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dc.contributor.authorThippeswamy S
dc.contributor.authorMulimani V.H.
dc.date.accessioned2020-06-12T15:08:13Z-
dc.date.available2020-06-12T15:08:13Z-
dc.date.issued2002
dc.identifier.citationProcess Biochemistry , Vol. 38 , 5 , p. 635 - 640en_US
dc.identifier.uri10.1016/S0032-9592(02)00010-9
dc.identifier.urihttp://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5549-
dc.description.abstract?-Galactosidase from Gibberella fujikuroi was immobilized in polyacrylamide gel. Free ?-galactosidase showed optimal activity at pH 5.8, immobilized ?-galactosidase showed at 5.2-5.6. In between pH 4.0 and 6.3, free and immobilized ?-galactosidase showed more than 90% activity. The optimum temperature of free and immobilized ?-galactosidase was 56°C and 54-56°C, respectively. Gibberella f. ?-galactosidase retained 100% activity after 6 h incubation at 55°C. After 12 h incubation ?-galactosidase retained 90% activity. After 3 days free enzyme retained only 6% initial activity and immobilized enzyme 17% initial activity. Soymilk was treated with free and immobilized ?-galactosidase from G. fujikuroi. After 3 h incubation raffinose family oligosaccharides were reduced to 79 and 66% by free and immobilized ?-galactosidase, respectively. Immobilized ?-galactosidase can be reused four times compared to free ?-galactosidase. A fluidized reactor was designed to treat soymilk continuously. Soymilk was passed through the fluidized reactor with different flow rates and maximum reduction in raffinose family oligosaccharides determined. © 2002 Elsevier Science Ltd. All rights reserved.en_US
dc.subjectAlpha galactosidase
dc.subjectFluidized reactor
dc.subjectGibberella fujikuroi
dc.subjectOligosaccharides
dc.subjectSoybean
dc.subjectSoymilk
dc.titleEnzymic degradation of raffinose family oligosaccharides in soymilk by immobilized alpha-galactosidase from Gibberella fujikuroien_US
dc.typeArticle
Appears in Collections:1. Journal Articles

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