A novel, rapid, inexpensive, and highly sensitive experiment for demonstration of DNA damage in human leukocytes by single cell gel electrophoresis (comet assay)

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DC Field	Value	Language
dc.contributor.author	Manjunatha B.R
dc.contributor.author	Manjunatha G.T.S
dc.contributor.author	Sarika C
dc.contributor.author	Roopa K.M
dc.contributor.author	Naik G.R.
dc.date.accessioned	2020-06-12T15:06:34Z	-
dc.date.available	2020-06-12T15:06:34Z	-
dc.date.issued	2003
dc.identifier.citation	Biochemistry and Molecular Biology Education , Vol. 31 , 1 , p. 34 - 36	en_US
dc.identifier.uri	10.1002/bmb.2003.494031010159
dc.identifier.uri	http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/5228	-
dc.description.abstract	The single cell gel electrophoresis technique (comet assay) has been adopted to demonstrate the DNA damage in human leukocytes of individuals who are habitual smokers. This technique allows the detection of single strand and double strand DNA breaks, which are indicative of the risk of cancer. The method followed is rapid, inexpensive, and a highly sensitive technique for the evaluation of DNA damage and risk assessment in smokers. This experiment should help students understand the effect of cigarette smoking. © 2003 by The International Union of Biochemistry and Molecular Biology.	en_US
dc.subject	Comet assay
dc.subject	DNA damage
dc.subject	Risk assessment
dc.subject	Smokers
dc.title	A novel, rapid, inexpensive, and highly sensitive experiment for demonstration of DNA damage in human leukocytes by single cell gel electrophoresis (comet assay)	en_US
dc.type	Article
Appears in Collections:	1. Journal Articles

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