Enhancement of Lipase Production from Pseudomonas sp by Induced Mutations

Abstract

Strain improvement program was developed to increase extracellular lipase production from Pseudomonas sp. The use of any scientific techniques that allows the isolation of cultures exhibiting a desired phenotype is the process of strain improvement. The utilization of improved microbes for industrial processes is not new. Today, the large scale production of health care products, amino acids, food additives, enzymes and antibiotics serves testimony to the important role of strain improvement in shaping the pharmaceutical and fermentation industries. The improvement of microbial strains offers the greatest opportunity for the cost reduction without significant capital investment. The purpose of the present investigation is to enhance the production of lipase by subjecting the indigenous lipase producing strain Pseudomonas aeruginosa RAS-4 to strain improvement by using UV light as a physical mutagen.