Development and validation of a high-performance liquid chromatographic determination of ceftriaxone sodium and its application to drug quality control

Abstract

A reliable and sensitive RP-HPLC method was developed and validated for the quantitative estimation of ceftriaxone sodium (CFTZ) in pure drug and pharmaceutical dosage forms. The separation of ceftriaxone sodium was achieved on a Waters XTerra RP-18 (5?m, 250 × 4.6mm i.d.) column using photodiode array detector at 240 nm. The mobile phase consisted of 0.1M triethylammoniumacetate-acetonitrile (60:40v/v) mixture delivered at a flow rate of 1.0 ml/min. Accuracy, evaluated by means of the spike recovery method, was excellent, with percent recovery in the range 99.5-102% with precision in the range 0.3-1.2%. © Taylor & Francis Group, LLC.