Spectrophotometric estimation of ganciclovir in bulk drug and its formulation

Abstract

Ganciclovir is an acyclic guanosine analog used in the treatment of AIDS. In present work, three new simple, sensitive, accurate and economical spectrophotometric methods have been developed for the estimation of Ganciclovir in bulk drug and its pharmaceutical formulation. Method A is the first order derivative spectroscopy method adopted to eliminate spectral interference, in which derivative amplitude was measured at 238 nm with n=1. Method B is based on calculation of area under curve for analysis of ganciclovir in wavelength range of 245-255 nm. The drug follows Beer's law in the concentration range of 5-25 ng/mL in both methods. In method C is based on reduction of the hetropoly acid, phosphomolybdotungstic acid, the well known Folin-Ciocalteu reagent in presence of alkali to form intense blue color chromogen exhibiting absorption maximum at 764.7nm and it obeyed Beer's law in the concentration range of 50-250 ng/mL. Results of the analysis were validated for accuracy, precision, LOD, LOQ and recovery studies and were found to be satisfactory. The proposed methods are simple, rapid and suitable for the routine quality control application.