Please use this identifier to cite or link to this item: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4155
Title: Invitro skin sensitization model
Authors: Gadwal R.
Keywords: Acute toxicity
CD80
Irritation
Skin sensitization
THP-1 cells
Issue Date: 2014
Publisher: International Journal of Pharma and Bio Sciences
Citation: International Journal of Pharma and Bio Sciences , Vol. 5 , 3 , p. B885 - B897
Abstract: The aim of this study was to develop a new in vitro skin sensitization model for predicting skin sensitizing potential of chemicals. We used THP-1, a human monocyte cell line for the optimization of human cell line activation test (h-CLAT) and conducted an inter-laboratory study by advanced molecular techniques. Sensitizers such as dinitrochlorobenzene (DNCB) enhanced expression of CD80, CD86 and other markers, while irritants such as sodium dodecyl sulfate (SDS) and dimethyl sulfoxide (DMSO) did not augment expression on THP-1 cells. A synergistic effect was observed when DNCB was added simultaneously to a culture. By evaluating the expression patterns of CD40, CD54, CD80 and CD86, we could classify the chemicals as sensitizers or nonsensitizers. These data suggest that THP-1 cells are good model for screening contact sensitizers and CD80 could be a useful marker complementary to CD54, CD86 and IL-?.
URI: http://gukir.inflibnet.ac.in:8080/jspui/handle/123456789/4155
Appears in Collections:1. Journal Articles

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