Antibacterial activity of biogenic zinc oxide nanoparticals synthesised from Enterococcus faecalis

Abstract

Zinc nanoparticles are known to be one of the multifunctional inorganic nanoparticles with effective antibacterial activity on both Gram negative and Gram positive bacteria. In the present research work, synthesis of zinc nanoparticles and its characterization was done. In this study, zinc nanoparticles were biologically synthesized from zinc sulfate solution using Enterococcus faecalis culture. The yellow color synthesized zinc nanoparticles were characterized by different spectroscopic and analytical techniques such as UV-Visible, Nanoparticle Tracking Analysis (NTA), Field emission Scanning Electron Microscopy (FeSEM) and Energydispersive X-ray (EDX). NTA and FeSEM analysis studies confirmed the formation of well-dispersed zinc nanoparticles with average particle size to be in the range of 16 to 96 nm as well as revealed their spherical structure. NTA analysis studies also confirmed the concentration of the particles in the range of 7.2×1010 particles/ml. These biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogens by using well diffusion method.